周光斌 更新日期:2018/6/11
周光斌,男,1974年出生,博士导师
工作单位 动物科技学院
电子邮箱 zguangbin@sicau.edu.cn
招生专业 博士:090501动物遗传育种与繁殖,学硕:090501动物遗传育种与繁殖,专硕:095133畜牧
◆个人简历
周光斌,男,汉族,中共党员,1974 年7 月生,教授,博士生导师,第九批、第十二批四川省学术和技术带头人后备人选,中国畜牧兽医学会动物繁殖学分会理事。
东北林业大学野生动物保护与利用专业1998届学士;中国农业大学动物遗传育种与繁殖专业2007届博士,主要从事动物胚胎/配子超低温冷冻保存机理与应用。2001年9月起在四川农业大学从事《胚胎工程》、《细胞工程》、《专业英语》、《动物繁殖学》、《动物遗传育种与繁殖学》及《低温生物学前沿》等教学和科研工作; 2007年5月至7月以访问科学家的身份在匈牙利农业生物技术中心从事三个月的合作研究,研究侧重于小鼠胚胎克隆及其胚胎移植;2008 年3 月至2011年2月在农业生物技术国家重点实验室从事博士后研究,开展的工作主要集中于猪胚胎干细胞建系及其体外诱导分化的研究。2014年8月至2015年8月在美国犹他州立大学访问学习,研究工作为"光照对仓鼠卵母细胞发育的影响"。
主持和参与国家及省部级科研项目多项,其中有中国博士后科学基金、国家转基因重大专项、国家自然科学基金、973和863项目。在《Cryobiology》、《Biology of reproduction》、《Animal biotechnology》、《Molecular human reproduction》和《中国兽医学报》等国内外刊物上发表学术论文61余篇(其中SCI收录45篇),主编或副主编或参编科学出版社专著和教材5部。
主要研究方向
1.动物(牛、羊和小鼠)卵母细胞/胚胎超低温冷冻及体外发育
2.转基因奶山羊安全评价
3.家畜(猪、牛和羊)胚胎干细胞建系及分化机制
◆工作经历
2013.12 - 今,四川农业大学,动物科技学院,教授
2014.08 – 2015.08,美国犹他州立大学,动物乳品和兽医科学系,访问学者
2009.12 – 2013.11,四川农业大学,旅游学院及动物科技学院,副教授
2008.03 – 2011.03,农业生物技术国家重点实验室,博士后
2007.05 – 2007.07,匈牙利农业生物技术中心,访问学者
2006.12 – 2009.11,四川农业大学,旅游学院,讲师
2001.09 – 2002.08,四川农业大学都江堰校区,助讲
1998.07 – 2001.08,四川省林业学校,助讲
◆教育经历
2002.09 – 2007.12,中国农业大学动物科技学院,动物遗传育种与繁殖系,攻读博士学位
1994.10 – 1998.7,东北林业大学野生动物资源学院,野生动物保护与利用,攻读学士学位
◆获奖荣誉
狐狸高效繁殖技术体系的研究与应用,2012.12获山东省科技进步三等奖,排名第四
◆社会、学会及学术兼职
中国畜牧兽医学会动物繁殖学分会理事
◆研究领域
1. 动物(牛、羊和小鼠)卵母细胞/胚胎超低温冷冻及体外发育

2. 转基因奶山羊安全评价

3. 家畜(猪、牛和羊)胚胎干细胞建系及分化机制
◆科研项目
1. Cd9表观遗传修饰影响超低温冷冻牛卵母细胞受精潜力的研究,国家自然科学基金面上项目(31572398),2016.1-2019.12,主持;

2. 强化褪黑素功能乳的转基因奶山羊新品种培育,国家科技重大专项(项目号:2014ZX08008-002B),2014.1-2016.12,二级子课题主持;

3. 奶牛高效繁殖技术集成与应用,国家奶牛产业技术体系,2015.1-2020.12,子课题主持
◆发表论文
1. Zhou G, Wei H, Wang X, Yang M, Bunch TD, Polejaeva IA, White KL, Wang Z and Meng Q (2018) Serial Culture Is Critical for In Vitro Development of Parthenogenetic Embryos in the Golden Syrian Hamster. Cell Reprogram 20:187-195. doi: 10.1089/cell.2017.0070
2. Ma Y, Pan B, Yang H, Qazi IH, Wu Z, Zeng C, Zhang M, Meng Q and Zhou G (2018) Expression of CD9 and CD81 in bovine germinal vesicle oocytes after vitrification followed by in vitro maturation. Cryobiology 81:206-209. doi: 10.1016/j.cryobiol.2018.02.011
3. Huang J, Ma Y, Wei S, Pan B, Qi Y, Hou Y, Meng Q, Zhou G and Han H (2018) Dynamic changes in the global transcriptome of bovine germinal vesicle oocytes after vitrification followed by in vitro maturation. Reprod Fertil Dev. doi: 10.1071/RD17535
4. Zhou G, Zeng Y, Guo J, Meng Q, Meng Q, Jia G, Cheng K, Zeng C, Zhang M, Liu G and Zhu S (2016) Vitrification transiently alters Oct-4, Bcl2 and P53 expression in mouse morulae but does not affect embryo development in vitro. Cryobiology 73:120-125. doi: 10.1016/j.cryobiol.2016.08.011
5. Zhang Y, Li W, Ma Y, Wang D, Zhao X, Zeng C, Zhang M, Zeng X, Meng Q and Zhou G (2016) Improved development by melatonin treatment after vitrification of mouse metaphase II oocytes. Cryobiology 73: 335–342. doi: 10.1016/j.cryobiol.2016.09.171
6. Chen XJ, Zhang Y, Jia GX, Meng QG, Bunch TD, Liu GS, Zhu SE and Zhou GB (2016) Effect of melatonin supplementation on cryopreserved sperm quality in mouse. Cryo Letters 37:115-22.
7. Li W, Cheng K, Zhang Y, Meng Q, Zhu S and Zhou G (2015) No effect of exogenous melatonin on development of cryopreserved metaphase II oocytes in mouse. J Anim Sci Biotechnol 6:42. doi: 10.1186/s40104-015-0041-0
8. Zhou Y, Fu X, Zhou G, Jia B, Fang Y, Hou Y and Zhu S (2014) An efficient method for the sanitary vitrification of bovine oocytes in straws. J Anim Sci Biotechnol 5:19. doi: 10.1186/2049-1891-5-19
9. Wang L, Zhuo ZY, Shi WQ, Tan DX, Gao C, Tian XZ, Zhang L, Zhou GB, Zhu SE, Yun P and Liu GS (2014) Melatonin Promotes Superovulation in Sika Deer (Cervus nippon). Int J Mol Sci 15:12107-18. doi: 10.3390/ijms150712107
10. Li Q, Wang H, Zhou GB, Jinfei M, Yan L, Zhaihu Z and Li N (2014) Production of healthy cloned pigs with neural stem cells as nuclear donors. Anim Biotechnol 25:294-305. doi: 10.1080/10495398.2013.872119
11. Cheng J, Jia B, Wu T, Zhou G, Hou Y, Fu X and Zhu S (2014) Effects of vitrification for germinal vesicle and metaphase II oocytes on subsequent centromere cohesion and chromosome aneuploidy in mice. Theriogenology 82:495-500. doi: 10.1016/j.theriogenology.2014.05.009
12. Zhou GB, Zeng Y, Meng QG, Liu Y, Dai YP, Zhu SE, Bunch TD and Hou YP (2013) Decreased expression of CD9 in bovine oocytes after cryopreservation and the relationship to fertilization capacity. Mol Reprod Dev 80:451-459. doi: 10.1002/mrd.22181
13. Zhou GB and Li N (2013) Bovine oocytes cryoinjury and how to improve their development following cryopreservation. Anim Biotechnol 24:94-106. doi: 10.1080/10495398.2012.755466
14. Zeng Y, Fu X, Zhou G, Yue M, Zhou Y and Zhu S (2013) Expression and distribution of cell adhesion-related proteins in bovine parthenogenetic embryos: The effects of oocyte vitrification. Theriogenology 80:34-40. doi: 10.1016/j.theriogenology.2013.03.006
15. Xia W, Fu XW, Zhou GB, Xu J, Wang L, Du M, Yuan DS, Yue MX, Tian JH and Zhu SE (2013) Cytokeratin distribution and expression during the maturation of mouse germinal vesicle oocytes after vitrification. Cryobiology 66:261-6. doi: 10.1016/j.cryobiol.2013.02.062
16. Zhang QJ, Zhou GB, Wang YP, Fu XW and Zhu SE (2012) Cryoprotectants protect medaka (Oryzias latipes) embryos from chilling injury. Cryo Letters 33:108-17.
17. Yue MX, Fu XW, Zhou GB, Hou YP, Du M, Wang L and Zhu SE (2012) Abnormal DNA methylation in oocytes could be associated with a decrease in reproductive potential in old mice. J Assist Reprod Genet 29:643-50. doi: 10.1007/s10815-012-9780-4
18. Wusiman A, Wang YP, Ren K, Zhou GB, Fu XW, Suo L, Fan ZQ, Wang L and Zhu SE (2012) Semen Storage at 23, 4 or -196°C and its Application to Artificial Insemination in Small-tail Han Sheep. Asian Journal of Animal and Veterinary Advances 7:299-308.
19. Wang L, Zhou GB, Shi WQ, Shi JM, Tian XZ, Gao C, Zhang L, Zhu SE, Zhang TT, Zeng SM and Liu GS (2012) First live offspring born in superovulated sika deer (Cervus nippon) after embryo vitrification. Theriogenology 78:1627-32. doi: 10.1016/j.theriogenology.2012.07.008
20. Gao C, Han HB, Tian XZ, Tan DX, Wang L, Zhou GB, Zhu SE and Liu GS (2012) Melatonin promotes embryonic development and reduces reactive oxygen species in vitrified mouse 2-cell embryos. J Pineal Res 52:305-11. doi: 10.1111/j.1600-079X.2011.00944.x
21. Wu GQ, Jia BY, Li JJ, Fu XW, Zhou GB, Hou YP and Zhu SE (2011) L-carnitine enhances oocyte maturation and development of parthenogenetic embryos in pigs. Theriogenology 76:785-93. doi: 10.1016/j.theriogenology.2011.04.011
22. Wang YP, Zhou GB, Zeng Y, Li JJ, Zhang QJ, Hou YP and Zhu SE (2011) Impact on Hyperactivated Motility of Cryopreserved Mouse Sperm from Pretreatment with Thimerosal. Asian Journal of Animal and Veterinary Advances 6:1052-1060.
23. Wang L, Liu J, Zhou GB, Hou YP, Li JJ and Zhu SE (2011) Quantitative investigations on the effects of exposure durations to the combined cryoprotective agents on mouse oocyte vitrification procedures. Biol Reprod 85:884-94. doi: 10.1095/biolreprod.110.090118
24. Li JJ, Pei Y, Zhou GB, Suo L, Wang YP, Wu GQ, Fu XW, Hou YP and Zhu SE (2011) Histone deacetyltransferase1 expression in mouse oocyte and their in vitro-fertilized embryo: effect of oocyte vitrification. Cryo Letters 32:13-20.
25. Fu XW, Wu GQ, Li JJ, Hou YP, Zhou GB, Lun S, Wang YP and Zhu SE (2011) Positive effects of Forskolin (stimulator of lipolysis) treatment on cryosurvival of in vitro matured porcine oocytes. Theriogenology 75:268-75. doi: S0093-691X(10)00441-3 [pii]
10.1016/j.theriogenology.2010.08.013
26. Zhou GB, Meng QG and Li N (2010) In vitro derivation of germ cells from embryonic stem cells in mammals. Mol Reprod Dev 77:586-594. doi: 10.1002/mrd.21187
27. Yan CL, Fu XW, Zhou GB, Zhao XM, Suo L and Zhu SE (2010) Mitochondrial behaviors in the vitrified mouse oocyte and its parthenogenetic embryo: effect of Taxol pretreatment and relationship to competence. Fertil Steril 93:959-966. doi: S0015-0282(08)04746-8 [pii]
10.1016/j.fertnstert.2008.12.045
28. Tian XZ, Wen Q, Shi JM, Liang W, Zeng SM, Tian JH, Zhou GB, Zhu SE and Liu GS (2010) Effects of Melatonin on In Vitro Development of Mouse Two-Cell Embryos Cultured in HTF Medium. Endocr Res 35:17-23. doi: 10.3109/07435800903539607
29. Zhou GB, Ma CB, Liu GS, Zhu SE, Zhang HH, Jia LL, Suo L, Shi JM, Wang YB, Tian JH and Zeng SM (2009) Vitrification of farmed blue fox oocytes in ethylene glycol and dmso-based solutions using open-pulled straw (OPS). Cryo Letters 30:112-8.
30. Zhou GB, Liu GS, Meng QG, Liu Y, Hou YP, Wang XX, Li N and Zhu SE (2009) Tetraspanin CD9 in bovine oocytes and its role in fertilization. J Reprod Dev 55:305-8. doi: JST.JSTAGE/jrd/20099 [pii]
31. Zhou GB and Li N (2009) Cryopreservation of porcine oocytes: recent advances. Mol Hum Reprod 15:279-85. doi: gap016 [pii]
10.1093/molehr/gap016
32. Suo L, Zhou GB, Meng QG, Yan CL, Fan ZQ, Zhao XM, Fu XW, Wang YP, Zhang QJ and Zhu SE (2009) OPS vitrification of mouse immature oocytes before or after meiosis: the effect on cumulus cells maintenance and subsequent development. Zygote 17:71-7. doi: S0967199408005091 [pii]
10.1017/S0967199408005091
33. Suo L, Wang F, Zhou GB, Shi JM, Wang YB, Zeng SM, Tian JH, Zhu SE and Liu GS (2009) Optimal concentration of calcium and electric field levels improve tetraploid embryo production by electrofusion in mice. J Reprod Dev 55:383-5. doi: JST.JSTAGE/jrd/20152 [pii]
34. Shi JM, Tian XZ, Zhou GB, Wang L, Gao C, Zhu SE, Zeng SM, Tian JH and Liu GS (2009) Melatonin exists in porcine follicular fluid and improves in vitro maturation and parthenogenetic development of porcine oocytes. J Pineal Res 47:318-23. doi: JPI717 [pii]
10.1111/j.1600-079X.2009.00717.x
35. Fu XW, Shi WQ, Zhang QJ, Zhao XM, Yan CL, Hou YP, Zhou GB, Fan ZQ, Suo L, Wusiman A, Wang YP and Zhu SE (2009) Positive effects of Taxol pretreatment on morphology, distribution and ultrastructure of mitochondria and lipid droplets in vitrification of in vitro matured porcine oocytes. Anim Reprod Sci 115:158-68. doi: S0378-4320(08)00490-9 [pii]
10.1016/j.anireprosci.2008.12.002
36. Yan CL, Yang QE, Zhou GB, Hou YP, Zhao XM, Fan ZQ, Liu MQ, Liu L and Zhu SE (2008) Open-pulled straw (OPS) vitrification of in vitro fertilised mouse embryos at various stages. Acta Vet Hung 56:245-53.
37. Wang YP, Zhao XM, Zhou GB, Hou YP, Fan ZQ, Yan CL, Suo L, Fu XW and Zhu SE (2008) Production of normal offspring from partially zona-incised vitrified mouse oocytes fertilized with cryopreserved spermatozoa using an optimized protocol. Cryo Letters 29:111-9.
38. Liu GS, Zhou GB, Zhang HH, Ma CB, Shi WQ, Zhu SE, Yang ZQ, Kang J, Jia LL, Zeng SM, Tian JH and Wang F (2008) Surgical embryo transfer resulted in birth of live offspring in farmed blue fox. Anim Reprod Sci 105:424-9. doi: S0378-4320(07)00418-6 [pii]
10.1016/j.anireprosci.2007.12.008
39. Fan ZQ, Li XW, Liu Y, Meng QG, Wang YP, Hou YP, Zhou GB and Zhu SE (2008) Piezo-assisted in vitro fertilization of mouse oocytes with spermatozoa retrieved from epididymides stored at 4 degree C. J Reprod Dev 54:107-12. doi: JST.JSTAGE/jrd/19118 [pii]
40. Zhou C, Zhou GB, Zhu SE, Hou YP, Jin F, Zhao XM and Hong QH (2007) Open-pulled straw (OPS) vitrification of mouse hatched blastocysts. Anim Biotechnol 18:45-54. doi: 770746316 [pii]
10.1080/10495390600826412
41. Zhao XM, Quan GB, Zhou GB, Hou YP and Zhu SE (2007) Conventional freezing, straw, and open-pulled straw vitrification of mouse two pronuclear (2-PN) stage embryos. Anim Biotechnol 18:203-12. doi: 779878711 [pii]
10.1080/10495390701201663
42. Yang ZQ, Zhou GB, Hou YP, Yan CL and Zhu SE (2007) Effect of in-straw thawing on in vitro- and in vivo-development of vitrified mouse morulae. Anim Biotechnol 18:13-22. doi: 770747241 [pii]
10.1080/10495390601091040
43. Yang QE, Hou YP, Zhou GB, Yang ZQ and Zhu SE (2007) Stepwise in-straw dilution and direct transfer using open pulled straws (OPS) in the mouse: a potential model for field manipulation of vitrified embryos. J Reprod Dev 53:211-8. doi: JST.JSTAGE/jrd/18045 [pii]
44. Tian SJ, Yan CL, Yang HX, Zhou GB, Yang ZQ and Zhu SE (2007) Vitrification solution containing DMSO and EG can induce parthenogenetic activation of in vitro matured ovine oocytes and decrease sperm penetration. Anim Reprod Sci 101:365-71. doi: S0378-4320(07)00004-8 [pii]
10.1016/j.anireprosci.2007.01.007
45. Zhou GB, Hou YP, Jin F, Yang QE, Yang ZQ, Quan GB, Tan HM and Zhu SE (2005) Vitrification of mouse embryos at various stages by open-pulled straw (OPS) method. Anim Biotechnol 16:153-63.
46. 周光斌, 曾艳, 孟庆勇, 侯云鹏 and 朱士恩 (2014) 超低温冷冻对牛卵母细胞组蛋白乙酰化和膜蛋白CD9表达的影响. 中国兽医学报 34:999-1004.
47. 周光斌, 将 郭, 艳 曾, 程柯仁, 傅祥伟 and 朱士恩 (2013) 超低温冷冻对小鼠桑椹胚多能性因子Oct-4表达的影响. 中国畜牧杂志 49:19-22.
48. 周光斌, 孟庆勇, 王弘, 李秋艳, 赵志辉 and 李宁 (2012) 猪孤雌胚胎干细胞建系的研究. 中国兽医学报 32:917-921.
49. 周光斌, 孟庆勇, 丁方荣, 戴蕴平, 李秋艳 and 李宁 (2012) ICR小鼠胚胎干细胞建系初步研究. 中国畜牧杂志 48:19-22,50.
50. 周光斌, 张清靖, 侯云鹏, 史文清 and 朱士恩 (2010) 哺乳动物胚胎玻璃化冷冻研究进展. 中国畜牧杂志 46:69-72.
51. 王晓旭, 周光斌, 李秀伟, 闫长亮 and 朱士恩 (2008) 冷冻后小鼠卵母细胞的透明带和质膜对体外受精的影响. 中国畜牧杂志 44:17-20.
52. 周光斌, 金方, 侯云鹏, 杨中强, 刘国世, 田见晖 and 朱士恩 (2006) 小鼠4-细胞胚胎OPS法冷冻保存技术. 中国兽医学报 26:104-106.
53. 周光斌, 侯云鹏, 杨中强, 杨其恩, 李秀伟 and 朱士恩 (2006) OPS两步法玻璃化冷冻小鼠8-细胞胚胎生存发育率的研究. 实验动物与比较医学 26:29-33.
54. 杨中强, 朱士恩, 周光斌, 侯云鹏 and 闫长亮 (2006) 不同冷冻和解冻方法对小鼠桑椹胚发育的影响. 中国畜牧杂志 42:10-12.
55. 杨其恩, 侯云鹏, 杨中强, 周光斌, 李秀伟 and 朱士恩 (2006) 不同温度条件下小鼠囊胚OPS法玻璃化冷冻保存技术的研究. 分子细胞生物学报 39:55-60.
56. 李秀伟, 吴通义, 侯云鹏, 周光斌 and 朱士恩 (2006) 小鼠卵母细胞的OPS法玻璃化冷冻保存技术. 中国比较医学杂志 16:55-60.
57. 胡辛怡, 周光斌, 杨中强, 刘国世 and 朱士恩 (2006) 小鼠4、8-细胞胚胎单卵裂球体外培养体系的研究. 中国畜牧杂志 42:15-18.
58. 赵学明, 朱士恩, 侯云鹏 and 周光斌 (2005) 双重套式PCR对不同取样胚胎性别鉴定灵敏度测试. 中国生物工程杂志 25:21-26.
59. 周崇, 侯云鹏, 周光斌, 吴通义, 金方, 洪琼花 and 朱士恩 (2004) 小鼠孵化囊胚细管法和OPS法玻璃化冷冻保存技术的研究. 中国农业大学学报 9:30-35.
60. 赵学明, 周光斌, 朱士恩, 曾申明 and 田见晖 (2004) OPS法冷冻小鼠胚胎的双重套式PCR性别鉴定. 中国畜牧杂志 40:30-32.
61. 金方, 侯云鹏, 周光斌, 赵学明 and 朱士恩 (2004) 小鼠胚胎徒手分割技术的研究. 中国实验动物学报 12:57-60.
◆专著教材
1. 《特种经济动物生产学》 科学出版社 第2版 (2018.2) 参编
2. 《动物配子与胚胎冷冻保存原理及应用》 科学出版社 第2版 (2016.11) 主编
3. 《动物克隆与基因组编辑》 中国农业大学出版社 第1版(2012.7)参编
4. 《动物配子与胚胎冷冻保存原理及应用》 科学出版社 第1版 (2012.2) 副主编
5. 《特种经济动物生产学》 科学出版社 第1版 (2009.5) 参编
◆教学活动
(1)动物科学专业本科生:《动物繁殖学》双语教学
(2)动物遗传育种与繁殖专业研究生:《动物遗传育种与繁殖学》
(3)国际合作交流生:《低温生物学前沿》